Tapered fibers combined with a multi-electrode array for optogenetics in mouse medial prefrontal cortex
Leonardo Sileo, Sebastian Bitzenhofer, Barbara Spagnolo, Jastyn Poepplau, Tobias Holzhammer, Marco Pisanello, Filippo Pisano, Elisa Bellistri, Emanuela Maglie, Patrick Ruther, Ileana L. Hanganu Opatz, Massimo De Vittorio, Ferruccio Pisanello
Optogenetic approaches offer huge advantages in spatial, temporal and cell-type specific control of neuronal activity. For that, a precise delivery of light achieved by optrodes is fundamental. In this article we compared a common used flat-cleaved optical fiber to a tapered optical fiber which aims a more uniform illumination of neurons along the dorso-ventral axis of the medial prefrontal cortex.