Methods

Electrophysiological recordings in vivo and in vitro

• Extracellular recordings (in vivo)
• Patch clamp recordings (in vivo & in vitro)

Optogenetics and chemogenetics in vivo and in vitro

• In utero electroporation
• Light manipulations
• Chemogenetics (DREADDs)

Neuroanatomy

• Morphological assessment
• Tracing of axonal projections
• Electron microscopy

Rodent behavior

• Neonatal vocalization
• Developmental milestones
• Open field
• Radial maze
• Object-recognition
• Multisensory testing

Data analysis

• Time-frequency analysis
• Local field potential coupling
• Single unit analysis
• Spike – LFP interactions
• Membrane properties